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sirius red fast green collagen staining kit  (Chondrex Inc)


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    Structured Review

    Chondrex Inc sirius red fast green collagen staining kit
    TRF reverses age-related fibrosis in adipose tissue. (A) Representative images of Masson’s Trichrome staining for collagen in adipose sections from young, aged, and aged-TRF animals. Black arrows point to blue-stained collagen fibers in adipose sections. (B) Image analysis from Trichrome staining showing % collagen-stained area in adipose sections. n = 5-6/group. (C) Semiquantitative analysis of collagen levels using <t>Sirius</t> <t>Red/Fast</t> Green staining after normalization to total protein levels. * p < .05, ** p < .01, *** p < .005, and **** p < .001 between the indicated groups. BAT, brown adipose tissue; eWAT, epididymal white adipose tissue; ingWAT, inguinal white adipose tissue; TRF, time-restricted feeding.
    Sirius Red Fast Green Collagen Staining Kit, supplied by Chondrex Inc, used in various techniques. Bioz Stars score: 96/100, based on 297 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/collagen+staining+kit/Sirius+Red%2FFast+Green+Collagen+Staining+Kit/pmc13120883-103-8-14
    Average 96 stars, based on 297 article reviews
    sirius red fast green collagen staining kit - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Time-restricted feeding improves metabolic flexibility, promotes beiging, and mitigates fibro-inflammation in the adipose tissue of aged mice"

    Article Title: Time-restricted feeding improves metabolic flexibility, promotes beiging, and mitigates fibro-inflammation in the adipose tissue of aged mice

    Journal: The Journals of Gerontology Series A: Biological Sciences and Medical Sciences

    doi: 10.1093/gerona/glag085

    TRF reverses age-related fibrosis in adipose tissue. (A) Representative images of Masson’s Trichrome staining for collagen in adipose sections from young, aged, and aged-TRF animals. Black arrows point to blue-stained collagen fibers in adipose sections. (B) Image analysis from Trichrome staining showing % collagen-stained area in adipose sections. n = 5-6/group. (C) Semiquantitative analysis of collagen levels using Sirius Red/Fast Green staining after normalization to total protein levels. * p < .05, ** p < .01, *** p < .005, and **** p < .001 between the indicated groups. BAT, brown adipose tissue; eWAT, epididymal white adipose tissue; ingWAT, inguinal white adipose tissue; TRF, time-restricted feeding.
    Figure Legend Snippet: TRF reverses age-related fibrosis in adipose tissue. (A) Representative images of Masson’s Trichrome staining for collagen in adipose sections from young, aged, and aged-TRF animals. Black arrows point to blue-stained collagen fibers in adipose sections. (B) Image analysis from Trichrome staining showing % collagen-stained area in adipose sections. n = 5-6/group. (C) Semiquantitative analysis of collagen levels using Sirius Red/Fast Green staining after normalization to total protein levels. * p < .05, ** p < .01, *** p < .005, and **** p < .001 between the indicated groups. BAT, brown adipose tissue; eWAT, epididymal white adipose tissue; ingWAT, inguinal white adipose tissue; TRF, time-restricted feeding.

    Techniques Used: Staining

    Related Articles

    Immunohistochemical staining:

    Article Title: Biomimetic Bilayer Scaffold as an Incubator to Induce Sequential Chondrogenesis and Osteogenesis of Adipose Derived Stem Cells for Construction of Osteochondral Tissue.
    Article Snippet: Towards osteochondral tissue construction, the present study introduced a bilayer scaffold to induce sequential chondrogenesis and osteogenesis of stem cells in vitro.. Two scaffolds that both based on poly (L-glutamic acid) (PLGA) and chitosan (CS) were combined to form the bilayer scaffold.. The cartilage region was the covalently crosslinked PLGA/CS hydrogel with tubular pore structure, possessing swollen network to prevent cellular adhesion, while induce spontaneous cellular aggregates formation.

    Article Title: Designer Hydrogel with Intelligently Switchable Stem-Cell Contact for Incubating Cartilaginous Microtissues.
    Article Snippet: Stem-cell-derived organoid can resemble in vivo tissue counterpart and mimic at least one function of tissue or organ, possessing great potential for biomedical application.. The present study develops a hydrogel with cell-responsive switch to guide adipose derived stem cells (ASCs) spontaneous and sequential proliferation and aggregation without inputting artificial stimulus for in vitro constructing cartilaginous micro-tissues with enhanced retention of cell-matrix and cell-cell interactions.. Polylactic acid (PLA) rods are surface-aminolyzed by cystamine, followed by being involved in the amidation of poly (L-glutamic acid) and adipic acid dihydrazide (ADH) to form a hydrogel.

    Staining:

    Article Title: Biomimetic Bilayer Scaffold as an Incubator to Induce Sequential Chondrogenesis and Osteogenesis of Adipose Derived Stem Cells for Construction of Osteochondral Tissue.
    Article Snippet: Towards osteochondral tissue construction, the present study introduced a bilayer scaffold to induce sequential chondrogenesis and osteogenesis of stem cells in vitro.. Two scaffolds that both based on poly (L-glutamic acid) (PLGA) and chitosan (CS) were combined to form the bilayer scaffold.. The cartilage region was the covalently crosslinked PLGA/CS hydrogel with tubular pore structure, possessing swollen network to prevent cellular adhesion, while induce spontaneous cellular aggregates formation.

    Article Title: Screening of MMP-13 Inhibitors Using a GelMA-Alginate Interpenetrating Network Hydrogel-Based Model Mimicking Cytokine-Induced Key Features of Osteoarthritis In Vitro
    Article Snippet: .. For immunohistochemistry (IHC) staining of type II collagen, antigen retrieval was performed by heating the sections in sodium citrate buffer (10 mM sodium citrate, 0.05% Tween 20, and pH 6.0) at 95 °C for 30 min. Then, type II collagen was stained using a Collagen Staining Kit (Chondrex), according to the manufacturer’s instructions. ..

    Article Title: Secretome Profiling of Young Multipotent Stem Cells Reveals Angiogenic and Immunomodulatory Mechanisms Supporting Aged Neuromuscular Health
    Article Snippet: .. Muscle fibrosis was assessed by double‐staining tissue sections with Sirius Red (collagen) and Fast Green (muscle tissue) using a collagen staining kit (Chondrex, #9046) for five minutes. ..

    Article Title: Three-dimensional Differentiated Human Mesenchymal Stem Cells Exhibit Robust Antifibrotic Potential and Ameliorates Mouse Liver Fibrosis
    Article Snippet: The liver tissues from mice were fixed with 4% paraformaldehyde for 1 day and embedded in Tissue-Tek OCT compound (Sakura Fine Technical Co. Ltd, Tokyo, Japan). .. Sectioned (10 μm thick) samples were stained with Harris hematoxylin solution (Sigma) for 3 min followed by eosin Y (Sigma) for 25 s. Collagen and hepatic fibrosis were assessed using a collagen staining kit (Chondrex, Inc., Redmond, WA, USA). ..

    Article Title: Designer Hydrogel with Intelligently Switchable Stem-Cell Contact for Incubating Cartilaginous Microtissues.
    Article Snippet: Stem-cell-derived organoid can resemble in vivo tissue counterpart and mimic at least one function of tissue or organ, possessing great potential for biomedical application.. The present study develops a hydrogel with cell-responsive switch to guide adipose derived stem cells (ASCs) spontaneous and sequential proliferation and aggregation without inputting artificial stimulus for in vitro constructing cartilaginous micro-tissues with enhanced retention of cell-matrix and cell-cell interactions.. Polylactic acid (PLA) rods are surface-aminolyzed by cystamine, followed by being involved in the amidation of poly (L-glutamic acid) and adipic acid dihydrazide (ADH) to form a hydrogel.

    Article Title: Secretome Profiling of Young Multipotent Stem Cells Reveals Angiogenic and Immunomodulatory Mechanisms Supporting Aged Neuromuscular Health.
    Article Snippet: .. Muscle fibrosis was assessed by double- staining tissue sections with Sirius Red (collagen) and Fast Green (muscle tissue) using a collagen staining kit (Chondrex, #9046) for five minutes. ..

    Immunohistochemistry:

    Article Title: Screening of MMP-13 Inhibitors Using a GelMA-Alginate Interpenetrating Network Hydrogel-Based Model Mimicking Cytokine-Induced Key Features of Osteoarthritis In Vitro
    Article Snippet: .. For immunohistochemistry (IHC) staining of type II collagen, antigen retrieval was performed by heating the sections in sodium citrate buffer (10 mM sodium citrate, 0.05% Tween 20, and pH 6.0) at 95 °C for 30 min. Then, type II collagen was stained using a Collagen Staining Kit (Chondrex), according to the manufacturer’s instructions. ..

    Double Staining:

    Article Title: Secretome Profiling of Young Multipotent Stem Cells Reveals Angiogenic and Immunomodulatory Mechanisms Supporting Aged Neuromuscular Health
    Article Snippet: .. Muscle fibrosis was assessed by double‐staining tissue sections with Sirius Red (collagen) and Fast Green (muscle tissue) using a collagen staining kit (Chondrex, #9046) for five minutes. ..

    Article Title: Secretome Profiling of Young Multipotent Stem Cells Reveals Angiogenic and Immunomodulatory Mechanisms Supporting Aged Neuromuscular Health.
    Article Snippet: .. Muscle fibrosis was assessed by double- staining tissue sections with Sirius Red (collagen) and Fast Green (muscle tissue) using a collagen staining kit (Chondrex, #9046) for five minutes. ..



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    TRF reverses age-related fibrosis in adipose tissue. (A) Representative images of Masson’s Trichrome staining for collagen in adipose sections from young, aged, and aged-TRF animals. Black arrows point to blue-stained collagen fibers in adipose sections. (B) Image analysis from Trichrome staining showing % collagen-stained area in adipose sections. n = 5-6/group. (C) Semiquantitative analysis of collagen levels using <t>Sirius</t> <t>Red/Fast</t> Green staining after normalization to total protein levels. * p < .05, ** p < .01, *** p < .005, and **** p < .001 between the indicated groups. BAT, brown adipose tissue; eWAT, epididymal white adipose tissue; ingWAT, inguinal white adipose tissue; TRF, time-restricted feeding.
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    Image Search Results


    TRF reverses age-related fibrosis in adipose tissue. (A) Representative images of Masson’s Trichrome staining for collagen in adipose sections from young, aged, and aged-TRF animals. Black arrows point to blue-stained collagen fibers in adipose sections. (B) Image analysis from Trichrome staining showing % collagen-stained area in adipose sections. n = 5-6/group. (C) Semiquantitative analysis of collagen levels using Sirius Red/Fast Green staining after normalization to total protein levels. * p < .05, ** p < .01, *** p < .005, and **** p < .001 between the indicated groups. BAT, brown adipose tissue; eWAT, epididymal white adipose tissue; ingWAT, inguinal white adipose tissue; TRF, time-restricted feeding.

    Journal: The Journals of Gerontology Series A: Biological Sciences and Medical Sciences

    Article Title: Time-restricted feeding improves metabolic flexibility, promotes beiging, and mitigates fibro-inflammation in the adipose tissue of aged mice

    doi: 10.1093/gerona/glag085

    Figure Lengend Snippet: TRF reverses age-related fibrosis in adipose tissue. (A) Representative images of Masson’s Trichrome staining for collagen in adipose sections from young, aged, and aged-TRF animals. Black arrows point to blue-stained collagen fibers in adipose sections. (B) Image analysis from Trichrome staining showing % collagen-stained area in adipose sections. n = 5-6/group. (C) Semiquantitative analysis of collagen levels using Sirius Red/Fast Green staining after normalization to total protein levels. * p < .05, ** p < .01, *** p < .005, and **** p < .001 between the indicated groups. BAT, brown adipose tissue; eWAT, epididymal white adipose tissue; ingWAT, inguinal white adipose tissue; TRF, time-restricted feeding.

    Article Snippet: The semiquantitative collagen detection kit for total tissue (Sirius Red/Fast Green collagen staining kit, Chondrex, Inc., catalog # 9046, Woodinville, WA) was used to assess the total amount of collagen and noncollagenous proteins in adipose tissue sections from the three experimental groups.

    Techniques: Staining